Pink or Blue®
is based on the latest gender testing technology. The test
is based on analysis of fetal DNA from the mother’s
Science is Proven...
- PCR detection of cell-free
circulating fetal DNA in maternal plasma and serum
(Lo et al. Lancet 1997; 350:485-7)
- Earliest detection of fetal
DNA: 5 weeks of gestation (Prenatal Diagnosis,
2003; 23: 1042)
- Source of fetal DNA? Passive
diffusion across placental barrier (van Wijk
et al. Clinical Chemistry, 2000; 46:729-731)
In a natural process, fetal cells die and pass into the mother’s
bloodstream. As these cells break down, fetal DNA is released
into the mother’s own circulatory system. Pink or Blue®
can detect the baby’s
DNA as early as 7 weeks into a pregnancy which is approximately
9 weeks from your last menstrual period. Pink or Blue®
have developed a process to determine the baby’s sex
based on analysis of the fetal DNA in the mother's bloodstream.
Here to Calculate
or Blue Eligibility Date.
The human Y-chromosome is only found in males. Pink or Blue®
looks for Y-chromosomal DNA in the mother’s blood using
a technique called Real-Time
Polymerase Chain Reaction (RT-PCR) that can amplify small
amounts of DNA to easily detectable levels. Pink or Blue®'s
patented technology is more sensitive than previous techniques
and thus we can provide an accurate
answer from three drops of your blood (average amount/drops
to fill one circle) on our DNA collection card. Although,
we can run a sample from one circle, we request that the expectant
mother fill all three circles. The DNA sample is tested twice
in parallel for quality assurance purposes. Y-DNA will only
be present in the sample if the baby is a boy, since the mother
does not carry the Y-chromosomal DNA herself. If no Y-DNA
is detected by Pink or Blue®, the mother is
expecting a baby girl.
DNA samples are analyzed in our lab using RT-PCR.
The classic study validating the PCR method for detecting
fetal sex was published in the Journal of Human Genetics
in 2005. Scientists in Italy (Galbiati et al) obtained blood
from pregnant women at different stages of pregnancy and
isolated fetal DNA as early as 5 weeks of pregnancy. The
isolated fetal DNA is amplified and analyzed using PCR.
They studied 1,837 pregnant women. This included women with
previous pregnancies, miscarriages and live births. The
test results were confirmed after the baby's birth and their
study had a 99% accuracy rate. Please note that the 99%
accuracy rate does not reflect Pink or Blue®'s
accuracy rate. Pink or Blue® is 95% accurate
based on an internal survey.
Pink or Blue® is highly accurate in determining
the presence of male DNA in the mother’s blood. However,
there are a few instances, such as a recent blood transfusion
or a bone marrow transplant. These situations, amongst others,
can cause Y-specific chromosomal DNA detection causing false
results. For more information, please see Is
Pink or Blue® Right for Me?
More Information About DNA Gender
Are you asking, "Am I having a Boy or Girl?"
The Early DNA Gender Test Has the Answer.
to finding out if you’re having a baby boy or
the Pink or Blue® Gender Test Kit online or over
2. Following the directions provided
in the Pink or Blue® Kit, use the 2 included lancets
to collect a finger prick blood specimen from the
3. Send the specimens to lab using
the pre-addressed, prepaid envelope included in the
4. Results are mailed, emailed and
available online within 3-5 days.